DAPI from BioLegend

Product Specs
ItemDAPI
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number422801
Quantity10 mg
ApplicationsIntracellular Staining for Flow Cytometry (ICFC), Immunocytochemistry (ICC)
FormatLyophilized
TargetDAPI

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Hyperactive Natural Killer cells in Rag2 knockout mice inhibit the development of acute myeloid leukemiaCommun BiolDecember 21, 2023Emi Sugimoto, Jingmei Li, Yasutaka Hayashi, Kohei Iida, Shuhei Asada, Tsuyoshi Fukushima, Moe Tamura, Shiori Shikata, Wenyu Zhang, Keita Yamamoto, et al.FlowJo software (Treestar, Inc., San Carlos, CA). 4′,6-diamidino-2-phenylindole (DAPI) (BioLegend, Cat#422801, 1:3000) was used to exclude dead cells. Antibodies used in this study are provided in Supplementary

(2) Mutant PIK3CA is a targetable driver alteration in histiocytic neoplasmsBlood AdvancesDecember 12, 2023Benjamin H. Durham, Oshrat Hershkovitz-Rokah, Omar I. Abdel-Wahab, Mariko Yabe, Young Rock Chung, Gilad Itchaki, Maayan Ben-Sasson, Vered A. Asher-Guz, David Groshar, Seyram A. Doe-Tetteh, et al.(clone M5/114.15.2, BioLegend, 107614, 1:200) following the manufacturer's protocol. DAPI (BioLegend, 422801, 1:1000) was used to exclude dead cells (supplemental Figure 1). Fluorescence-activated cell sorter

(3) IL-17A-driven psoriasis is critically dependent on IL-36 signalingFront ImmunolDecember 11, 2023Berenice Fischer, Tanja Kübelbeck, Antonia Kolb, Julia Ringen, Ari Waisman, Miriam Wittmann, Susanne Karbach, Stephan Marcus Kölsch, Daniela KramerCat.550085, PE Hamster IgG2, κ, BD). For live/dead staining the samples were co-stained with DAPI (Cat.422801, BioLegend) or Zombie Green fixable viability kit (Cat.423111, Biolegend). Cell populations were gated

(4) Loss of Slc35a2 alters development of the mouse cerebral cortexbioRxivDecember 1, 2023Soad Elziny, Sahibjot Sran, Hyojung Yoon, Rachel R. Corrigan, John Page, Amanda Ringland, Anna Lanier, Sara Lapidus, James Foreman, Erin L. Heinzen, et al.were washed with 0.1M phosphate buffered saline (PBS), counterstained with DAPI (1:1000, BioLegend, 422801) and mounted on glass slides using Prolong Gold Antifade Mountant (Invitrogen, P36930). Image analysis One

(5) Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreasSTAR ProtocolsDecember 1, 2023Laura Steiger, Zora Baumann, Lena Keller, Marianne Böni-Schnetzler, Marc Y. Donath, Daniel T. Meiersimilar to Zombie NIR and can only be unmixed by spectral flow cytometry).Alternatives: Use DAPI (Cat # 422801, BioLegend) for live/dead staining. Add DAPI (1.38 μg/mL (3 μM)) immediately before acquiring samples

(6) Examining PI3K-signaling-dependent regulation of lens organelle free zone formation via immunolocalization and immunoblotting in chick embryosSTAR ProtocolsDecember 1, 2023Rifah Gheyas, A. Sue MenkoInc",N66001G Ethanol,Decon Labs,2701 Dulbecco’s phosphate-buffered salt solution (DPBS),Corning,MT21030CV DAPI,BioLegend,422801 Fetal bovine serum,Gibco,A31605-01 Medium 199,Gibco,11150-059 L-Glutamine,Corning,25-005-Cl Penicillin-streptomycin,Corning,30-002-Cl ProLong

(7) Antibody landscape of C57BL/6 mice cured of B78 melanoma via a combined radiation and immunocytokine immunotherapy regimenFrontiers in ImmunologyNovember 23, 2023Anna Hoefges, Sean J. McIlwain, Amy K. Erbe, Nicholas Mathers, Angie Xu, Drew Melby, Kaitlin Tetreault, Trang Le, Kyungmann Kim, Richard S. Pinapati, et al.with 3ml flow buffer and resuspended in 50-100μl flow buffer. A drop of DAPI (BioLegend, catalog # 422801) was added to each tube before data was acquired on a ThermoFisher Attune flow cytometer. Data analysis

(8) HPV16E1 downregulation altered the cell characteristics involved in cervical cancer developmentScientific ReportsOctober 25, 2023Thanayod Sasivimolrattana, Arkom Chaiwongkot, Parvapan Bhattarakosol(#ab6717; Abcam, USA) for another hour at 37 °C. Then, the slide was stained with 300 nM DAPI solution (#422801; Biolegend, USA) for 5 min at room temperature. Then, the slide was treated with anti-fade fluorescence

(9) The involvement of caspases in the process of nuclear removal during lens fiber cell differentiationCell Death DiscoveryOctober 21, 2023Rifah Gheyas, A. Sue Menko111-545-003) were from Jackson ImmunoResearch Laboratories. Nuclei were labeled with the DAPI reagent (422801) from Biolegend. Fluorescent tagging of the CAD antibody Before tagging the antibody with a fluorophore,

(10) Effector memory T cells induce innate inflammation by triggering DNA damage and a non-canonical STING pathway in dendritic cellsCell ReportsOctober 3, 2023Hannah E. Meibers, Kathrynne A. Warrick, Andrew VonHandorf, Charles N. Vallez, Kiana Kawarizadeh, Irene Saha, Omer Donmez, Viral G. Jain, Leah C. Kottyan, Matthew T. Weirauch, et al.(AZD6738),Selleck Chem,S7693 2-Mercaptoethanol (BME),Sigma,M6250 "4’,6-diamidino-2-phenylindole (DAPI)",Biolegend,422801 Paraformaldehyde aqueous solution (PFA),Fisher,15710 Fetal calf serum (FCS),Sigma,F0926 RBC lysis

(11) Generation of Novel Immunocompetent Mouse Cell Lines to Model Experimental Metastasis of High-Risk NeuroblastomaCancers (Basel)September 23, 2023Mayura R. Dhamdhere, Dan V. Spiegelman, Lisa Schneper, Amy K. Erbe, Paul M. Sondel, Vladimir S. Spiegelmanmin at RT in the dark. Following a washing step (300× g for 5 min), a drop of DAPI (BioLegend, Cat # 422801) was added to the cell suspensions, and data were acquired using a ThermoFisher Attune NxT flow cytometer.

(12) Let-7 enhances murine anti-tumor CD8 T cell responses by promoting memory and antagonizing terminal differentiationNature CommunicationsSeptember 11, 2023Alexandria C. Wells, Kaito A. Hioki, Constance C. Angelou, Adam C. Lynch, Xueting Liang, Daniel J. Ryan, Iris Thesmar, Saule Zhanybekova, Saulius Zuklys, Jacob Ullom, et al.cells were excluded by staining with 4′,6-diamidino-2-phenylindole, dilactate (DAPI, Biolegend, Cat# 422801). The following monoclonal antibodies from BioLegend were used: CD8α-Pacific Blue (1:20 dilution, clone

(13) Protocol for enrichment, purification, and cytocentrifugation of mouse liver endothelial cellsSTAR ProtocolsSeptember 1, 2023Shanin Chowdhury, Kaela Drzewiecki Fried, Yasuko Iwakiri, Joseph Brancale, Sílvia VilarinhoChemicals,Cat#12125-02-9 Fetal bovine serum (FBS),Gibco,Cat#10437-028 DAPI (1:100),BioLegend,Cat#422801 "PBS (10×), pH 7.4",Thermo Fisher Scientific,Cat#70011069 "PBS (1×), pH 7.4",Thermo Fisher Scientific,Cat#10010023 Percoll,Sigma-Aldrich,Cat#17-0891-02 Prolong™

(14) Epigenetic neural glioblastoma enhances synaptic integration and predicts therapeutic vulnerabilitybioRxivAugust 7, 2023Richard Drexler, Robin Khatri, Thomas Sauvigny, Malte Mohme, Cecile L. Maire, Alice Ryba, Yahya Zghaibeh, Lasse Dührsen, Amanda Salviano-Silva, Katrin Lamszus, et al.fixed with 4% PFA (Sigma/Aldrich), blocked with PBS containing 5% FBS, 0.1%TritonX and DAPI (4 ug/mL, #422801, Biolegend) for one hour at room temperature and were stained with vimentin (#677809, Biolegend), cleaved

(15) iPSC-derived reactive astrocytes from patients with multiple sclerosis protect cocultured neurons in inflammatory conditionsJournal of Clinical InvestigationJuly 3, 2023Janis Kerkering, Bakhrom Muinjonov, Kamil Sebastian Rosiewicz, Sebastian Diecke, Charlotte Biese, Juliane Schiweck, Claudia Chien, Dario Zocholl, Thomas Conrad, Friedemann Paul, et al.1:1,000; all from Invitrogen) were given for 1 hour, and cells were counterstained with DAPI (1:4,000; 422801, BioLegend); for mounting, DAKO Mounting medium was used. Images were taken on a Leica DMI6000 B fluorescence microscope

(16) Purification and functional characterization of novel human skeletal stem cell lineagesNature ProtocolsJuly 1, 2023Malachia Y. Hoover, Thomas H. Ambrosi, Holly M. Steininger, Lauren S. Koepke, Yuting Wang, Liming Zhao, Matthew P. Murphy, Alina A. Alam, Elizabeth J. Arouge, M. Gohazrua K. Butler, et al.(Sigma-Aldrich, cat. no. C0775) 4,6-Diamidino-2-phenylindole (DAPI) (10 mg) (BioLegend, cat. no. 422801) ▲CRITICAL Since DAPI is photosensitive, be sure to minimize exposure to light sources. Deoxyribonuclease

(17) Immune Cells Localize to Sites of Corneal Erosions in C57BL/6 MiceBiomoleculesJune 29, 2023Le PM, Pal-Ghosh S, Menko AS, Stepp MA.temperature before staining with primary antibodies for 2.5 h at 37 °C. Nuclei were labeled with DAPI (#422801, Biolegend, San Diego, CA, USA) for 30min before sections were washed and mounted in Prolong Diamond Antifade Mountant

(18) Infection with hypervirulent Mycobacterium tuberculosis triggers emergency myelopoiesis but not trained immunityFrontiers in ImmunologyJune 13, 2023Ana Raquel Maceiras, Diogo Silvério, Rute Gonçalves, Marcos S. Cardoso, Margarida Saraivaconjugated (Cat. #A-31573, Invitrogen) for 2 h at room temperature. Slides were stained with DAPI (Cat. #422801, Biolegend; 1:1000 dilution), and glass coverslips were mounted using Fluoroshield (Cat. #F6182, Sigma-Aldrich).

(19) IEC-intrinsic IL-1R signaling holds dual roles in regulating intestinal homeostasis and inflammationJournal of Experimental MedicineJune 5, 2023Garrett R. Overcast, Hannah E. Meibers, Emily M. Eshleman, Irene Saha, Lisa Waggoner, Krupaben N. Patel, Viral G. Jain, David B. Haslam, Theresa Alenghat, Kelli L. VanDussen, et al.Target,Clone,Company,Ref # Mouse E-Cadherin,4A2,CST,14472 DAPI,N/A,BioLegend,422801 CD45.2 FITC,104,BioLegend,109806 CD90.2 BV785,30-H12,BioLegend,105331 CD11b PE,M1/70,BioLegend,101208 Ly6C

(20) Elastin-targeted nanoparticles delivering doxycycline mitigate cytokine storm and reduce immune cell infiltration in LPS-mediated lung inflammationPLOS ONEJune 2, 2023Shivani Arora, Narendra Vyavahare(I-A/I-E)-PE-Cy7 (cat#25-5321-82, Invitrogen), Anti mouse Siglec F-PE (Cat#12-1702-80, Invitrogen), DAPI (Cat#422801, Biolegend), TruStainFcX Plus (Cat#156604, biolegend), Anti mouse CD11b-APC (Cat# 101212, biolegend),

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(1)

3/28/2023Great DAPI for Immunocytochemistry Umut TankI use it for nuclear staining of HEK293 and HeLa cells. Good brightness and super easy to use it, also the amount needed is really really low. So, it will last forever.
(1)

7/12/2022DAPI Nuclear Stain Matthew RatsepI use this reagent for routine staining of nuclei in immunofluorescence. It works great in most samples and gives a bright clear signal.

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DAPI from BioLegend

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